rabbit cd34 pab  (Servicebio Inc)

Journal: Journal of Traditional Chinese Medicine

Article Title: Saponin Ⅰ from Shuitianqi ( Rhizoma Schizocapasae Plantagineae ) inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg’s Albino/c mouse model

doi: 10.19852/j.cnki.jtcm.2024.04.006

Figure Lengend Snippet: A: Western blotting was used to detect the expression of CD31, CD34 and VEGF proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of CD31, CD34 and VEGF proteins expression. C: immunohistochemical assay was used to detect the expression of CD31 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of CD34 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: immunohistochemical assay was used to detect the expression of VEGF protein in model group, cisplatin group and SSPH Ⅰ groups. E1: model group; E2: cisplatin group; E3: SSPH Ⅰ (25 mg/kg) group; E4: SSPH Ⅰ (50 mg/kg) group; E5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. F: quantitative analysis of CD31, CD34 and VEGF proteins expression. G: enzyme linked immunosorbent assay was used to detect the expression of VEGF protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); VEGF: vascular endothelial growth factor; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.

Article Snippet: Subsequently, sections were incubated in 5% BSA blocking fluid for 1 h, with subsequent ON incubation in primary antibodies at 4 ℃: E-cadherin, N-cadherin, Vimentin, TGF-β1(PTG, 21898-1-AP), Smad7, MMP-2, MMP-9, CD31, CD34 (Sino Biological Inc., 50589-T24), and VEGF (all dilution 1:200).

Techniques: Western Blot, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Saline, Injection, Standard Deviation

Journal: Frontiers in Pediatrics

Article Title: PROX1-associated lymphatic reprogramming signatures in pediatric adamantinomatous craniopharyngioma: a comparative study with adult cases

doi: 10.3389/fped.2026.1865684

Figure Lengend Snippet: Spatial distribution and co-expression of endothelial and lymphatic markers in pediatric ACP tissues. Representative IHC staining of serial sections from pediatric ACP tissues demonstrating the spatial distribution of CD34, PROX1, and LYVE1. All three markers display overlapping expression that clearly delineates the same ring-like structures, indicating a distinct lymphatic phenotype within the tumor microenvironment. Scale bar, 50 µm.

Article Snippet: For IHC, primary antibodies against PROX1 (Rabbit mAb, ABclonal, Wuhan, China), Nestin (Rabbit mAb, ABclonal), CD34 (Rabbit PolymAb, ABclonal), and LYVE1 (Rabbit mAb, ABclonal) were employed, followed by incubation with appropriate secondary antibodies (Zhongshan Golden Bridge Biotechnology, Beijing, China).

Techniques: Expressing, Immunohistochemistry

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: Transcriptome-wide m6A Methylation Profiling Reveals Regulatory Networks During Endothelial Differentiation of Bone Marrow Mesenchymal Stem Cells

doi: 10.1177/10760296261472670

Figure Lengend Snippet: Isolation and characterization of human bone marrow-derived mesenchymal stem cells. (A) BM-MSCs exhibited a long, spindle form and showed a whirlpool-shaped growth pattern. (B) Characterization of cell surface markers in BM-MSCs at passage 3 by flow cytometry. BM-MSCs were positive for CD105, CD90, and CD29 but negative for CD31, CD34, and CD45

Article Snippet: After 10 days of culture in DMEM (uninduced group) or inducing medium (induced group), cells were seeded into 24-well chambers, washed with PBS, fixed with 4% paraformaldehyde for 15 min, and permeabilized with 0.5% Triton X-100 for 30 min. After 1 h of blocking with 3% bovine serum albumin (BSA), cells were incubated with rabbit anti-CD31 (1:20, Abcam, Cambridge, UK) and rabbit anti- CD34 (ABclonal, Wuhan, China) antibodies in a humidified chamber at 4 °C overnight.

Techniques: Isolation, Derivative Assay, Flow Cytometry

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: Transcriptome-wide m6A Methylation Profiling Reveals Regulatory Networks During Endothelial Differentiation of Bone Marrow Mesenchymal Stem Cells

doi: 10.1177/10760296261472670

Figure Lengend Snippet: Endothelial differentiation of BM-MSCs is confirmed by phenotypic and functional assays. (A) Flow cytometry showed an increased CD31+ cell proportion and CD34+ cell proportion after induction. (B) Western blot revealed that the expression of VE-cadherin increased after 10 days induction. (C) CD31 and CD34 immunostaining of induced cells and uninduced cells. Nuclei were stained with DAPI (blue). The expression of CD31 and CD34 were detected in induced groups. Scale bar = 200um. (D) Induced MSCs take up acetylated LDL. Scale bar = 200 um. Blue fluorescence signifies DAPI and red indicates DiI-labeled-acetylated LDL uptake in cells. (E) Tube formation assay: induced MSCs form a robust capillary-like network on Matrigel after 6 h. Scale bar = 1 mm

Article Snippet: After 10 days of culture in DMEM (uninduced group) or inducing medium (induced group), cells were seeded into 24-well chambers, washed with PBS, fixed with 4% paraformaldehyde for 15 min, and permeabilized with 0.5% Triton X-100 for 30 min. After 1 h of blocking with 3% bovine serum albumin (BSA), cells were incubated with rabbit anti-CD31 (1:20, Abcam, Cambridge, UK) and rabbit anti- CD34 (ABclonal, Wuhan, China) antibodies in a humidified chamber at 4 °C overnight.

Techniques: Functional Assay, Flow Cytometry, Western Blot, Expressing, Immunostaining, Staining, Fluorescence, Labeling, Tube Formation Assay